Re: HYB: genetics - luminata - pl-lu, pl-a, and pl - part 2
iris@hort.net
  • Subject: Re: HYB: genetics - luminata - pl-lu, pl-a, and pl - part 2
  • From: L* M* <l*@lock-net.com>
  • Date: Sun, 28 Oct 2012 13:01:06 -0400
Irisdude Discussions When you test this flower are you talking about selfing it and growing out the seedlings to see if there are any glaciatas that appear? Then crossing the seedlings with each other to see if their children produce any glaciatas?
Chuck Chapman Test crosses for genetics, involve crossing to a homozygous recessive. So it would involve crossing to a glaciata. As these are tetraploids, it would then require a second level of testing by crossing the F1 to a glaciata. Selfing could also be done to see what consistency of F1 would be, but it wouldn't be conclusive as to geneotype, unless it produced glaciatas , which would eliminate it as being 4 pl-lu. In all cases it would require a good number to enable a chi-square test of probability of fit to predicted phenotypes. If in F1 of test cross of this seedling to a glaciata , we got a glaciata, then there would be no need for a chi-square test or further testing.
Chuck Chapman Oh, and the glaciata used as a test cross should also be homozygous recessive for Ae, that is 4 x ae.
Virginia Spoon Okay, I'm back and Don has explained it to me. He wrote an article explaining all this in the Bulletin a few years ago titled "The Alleles of the plicata Locus." He also wrote a pretty good explanation in the Reblooming Iris Recorder in the last issue and it is on our website under information. I will try and explain it although it will be secondhand because Don is busy planting seeds before the Frankenstorm. Look at the two photos, Daughter of Stars and the one of our seedling: Daughter of Stars is much lighter with a white area around the beards and a lighter edge around the falls: From the plicata locus it has two doses of luminata (lu lu gl gl) and two of glaciata. The seedling has no white area around the beards and no white edge and is altogether much darker (lu lu lu lu). We have gotten glaciatas out of luminata crosses, about 2 out of a hundred. (gl gl gl gl). Presby's Crown Jewel and Spinning Wheel are (pl pl gl gl) as the have a clear white ground and a white border. Drama Queen is a Fanciata (pl pl lu lu) as is Epicenter. That's as far as I'm going on this.
Chuck Chapman So based on its phenotype it is seen as a 4x pl-lu. Hopefully it is, but I'll need to see appropriate test crosses to accept that. I need to see appropriate test cross of all plicata iris (which include a glaciata with 4 x ae, as there is an interaction) before being sure of it's pl allelic combination. A third factor, which may play a role in phenotype is the inclusion of center pattern versus exclusion. for example, Stepping Out is an exclusion ( no anthocyanin in center of flower petal) while Ring Around Rosie is strictly an inclusion plicata. these can combine to give iris having both inclusion and exclusion, such as Circus Stripes. And then there is dots versus stripes. Not an easy task to sort all this out.
Irisdude Discussions All this pl-lu-ga talk is fascinating! I love learning about this stuff ever since Don's article in the bulletin about Clarence. Chuck you have even further piqued my interest now with this thread. I wish I had the space to devote to the type of testing you are discussing. If you will notice my profile picture is one of the seedlings from my first cross of a PL X LU phenotype. Ever since then I have been hooked!
Chuck Chapman I have been working for a number of years to get a good test glaciata. So far I have a number of glaciatas. They are very tender in my climate, but I have a few promising ones. Then they need to be test crossed with a blue iris that is 4 x ae in order to find the ones that are 4 x ae. 

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